A Study at Siriraj Hospital

Solid-phase immunosorbent hemadsorption (SPIHAtl) for specific IgM detennination has been introduced for rubella serodiagnosis in combination with hemagglutination inhibition test (HAl) during the outbreak prevaDed from September 1983 to August 1984 with its peak in March. By HAl test, 92.7% of patients with apparent rubella infection whose first blood was obtained within 3 days after rash showed seroconversion as compared to their paired sera bled 7 days later. The remaining cases already developed high HAl titer (~80) and diagnosiswas done by further investigation for specific IgM. Rubella IgM determination by SPIHAd was valid for sera drawn within 4-5 weeks after rash or 8 weeks after contact with the index cases. During the epide­ mic, both tests have diagnosed clinical rubella in 70.0% of the patients with maculopapular erup­ tion, and subclinical infection in 5.1% of the contact cases. "" , onflO'l!jl ~h -3VWl1Jla l1tNfltJ ms~'l;)iih);lfJ1nnr~HfJtl' i'uYIl,nl'tl-3t.1ijtMon1um-y-3 ~-3m, 11: ...... fl"l'1J it1ari'u-i vt1i19)lU~. ;)UYlvt..1il 1~O. f11n11W l~iivnuu". U'~IO;G Y1f1..1Ii1;W • • .. u mFa'll1f}tI~,}Jntn FlflJ::ll',.mlJFf1iJ~fi1h1'l1WlJ11J1t1 J:/'ff1JnlJ1t1lJJJiifltl , .­ ~ ~ ~ v ~ n'jiJ1UiFI~fIl'llf)lltl::lJ1mj.J'ItI'lIW 2528; 2:131-135. f1W~e!i;)U1~'\.ilm1Vlnil"'1 rubella specific IgM l~uiii solid-phase immunosorbent hemadsorption (SPlHAdr Jn1,}'·h1.ltluiii hemagglutination inhibition (HAl) 1~f1il1i1uuhfl .... ....,' ~ ,.. u .. .. .... ..\... k ,..j "'~IUfI'1.IU WIJ1..1m11~Ul~"I..1I1V1I~f1UOUU1UUvt.ft. 2526 tHI~f1UO..1"'lfl1.l vt.ft. 2527 '11..11.1 pea f1~Y1 ... ... 'IIIllo f! 1 it 'iI_'1 U U .... <II ,........ 1~f1U1.IUlfl1.l iIl0mnlfl11~"'Ha"UfI..1HAl ~vtU11Hu1U"'~IUfI'1.IUYl1.Illill~lafl~IllU 31U",a..1 .. .. ... 1!11!11' ..... "'J'" !II 1 !II "'!II !II f1f10HUiI~vtU seroconversion ~'f1ua~ 92.7 01Uflmflua~ 7.3 1.IIIflUVlUfl~U"UU~ ..1 IIa1V1f1..1 ",m, iiiiluul~um'V1nil"'lspecific IgM fll HAl titer ~ 80 ;)~lilu1V1IV1f1~~..1lvt'1~ convalescent HAl titer ~fll~lO~~ 80 • ~~ ~ u f! u .. ... u f! u Rubella IgM i1~V1nilvtu1u'll'1.IYlliIl~1ul1al 4'5 oiJ~1M",a..1f1f10HU "',f1 8 oiJ~1M",a..1 D1.IHOhfl m,1,}'lYIf1'iiflSPlHAd h1.lo.... u HAl 'h1,fm,il1i1uuhfl~fI111.l11IYi1.l~U 1~u1u,~",iH 1 n!II .. .. ....' !II}l 1 !II "'!II .......... ...II 1 ' m11~Ul~ ~ ",mnUilnU11Hu1U "UflflOHUlfIUa~70.0 1O~i110"'~IUfI'1.IUlla~m'V1~I'IIflIIUU1.1 .. .. _I "IIllo ... it u'lO{JfIlm'IO~"UU'f1Ua~5.1 DeptJrtment ofMicrobiololfY, Faculty ofMedicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.